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M9460603.TXT
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1994-06-25
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Document 0603
DOCN M9460603
TI The multimerization state of retroviral RNA is modulated by ammonium
ions and affects HIV-1 full-length cDNA synthesis in vitro.
DT 9408
AU Weiss S; Hausl G; Famulok M; Konig B; Institut fur Biochemie,
Universitat Munchen, Germany.
SO Nucleic Acids Res. 1993 Oct 25;21(21):4879-85. Unique Identifier :
AIDSLINE MED/94232812
AB Genomic human immunodeficiency virus type 1 (HIV-1) RNA fragments
containing the dimer linkage structure (DLS) can be dimerized and
multimerized in the presence of NH4+ and in the absence of any other
cation and any viral or cellular protein. This effect strongly supports
the notion that dimerization and multimerization of genomic RNA occurs
via purine-quartet formation in quadruple helical RNA structures. The
efficiency of RNA dimerization and multimerization in the presence of
ammonium ions is about 400 fold increased as compared to alkali metal
ions such as potassium. Dimerized retroviral RNA representing a
pseudodiploid genome could account for genetic recombination within the
virion and during reverse transcription. Application of a novel
South-Northern-Blotting procedure with biotinylated RNA and
digoxigenin-labelled cDNA in vitro reveals that efficient human- and
bovine tRNA(Lys3) primed full-length cDNA-synthesis only takes place
with a predominantly monomerized RNA template. Dimerization and
multimerization of the RNA significantly reduces full-length
cDNA-synthesis. This suggests that monomerization of the dimerized RNA,
effected by deionization in vitro, is essential for efficient retroviral
reverse transcription in vivo.
DE Acetates/CHEMISTRY Ammonium Compounds/*CHEMISTRY Biopolymers/CHEMISTRY
Biotin Blotting, Northern/METHODS Blotting, Southern/METHODS
Digoxigenin DNA, Viral/*BIOSYNTHESIS/CHEMISTRY HIV Long Terminal
Repeat HIV-1/*GENETICS Ions Retroviridae/*GENETICS RNA,
Viral/*CHEMISTRY JOURNAL ARTICLE
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).